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abhd2 polyclonal antibody  (Proteintech)


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    Proteintech abhd2 polyclonal antibody
    Abhd2 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/abhd2 polyclonal antibody/product/Proteintech
    Average 93 stars, based on 10 article reviews
    abhd2 polyclonal antibody - by Bioz Stars, 2026-03
    93/100 stars

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    Image Search Results


    (A) Protein and (B) mRNA levels of ABHD2 and ADAM15 in parental, mock, and Casp9 KO MC3T3-E1 cells; α-tubulin was used as a loading control for immunoblotting. Data represents means ± SD from at least three independent experiments.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: (A) Protein and (B) mRNA levels of ABHD2 and ADAM15 in parental, mock, and Casp9 KO MC3T3-E1 cells; α-tubulin was used as a loading control for immunoblotting. Data represents means ± SD from at least three independent experiments.

    Article Snippet: Consecutive sections were pretreated in citrate buffer (10 min/98 °C) for antigen retrieval and then incubated with ABHD2 antibody (14039-1-AP, Proteintech, Germany) or antibody specific to cleaved CASP-9 (9509, Cell Signaling Technology, USA) overnight.

    Techniques: Control, Western Blot

    Protein levels of ABHD2 and ADAM15 in MC3T3-E1 cells with inhibited CASP-9 or CASP-3/-7; α-tubulin was used as a loading control.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Protein levels of ABHD2 and ADAM15 in MC3T3-E1 cells with inhibited CASP-9 or CASP-3/-7; α-tubulin was used as a loading control.

    Article Snippet: Consecutive sections were pretreated in citrate buffer (10 min/98 °C) for antigen retrieval and then incubated with ABHD2 antibody (14039-1-AP, Proteintech, Germany) or antibody specific to cleaved CASP-9 (9509, Cell Signaling Technology, USA) overnight.

    Techniques: Control

    Immunofluorescent staining of ABHD2 (A, C) and cleaved caspase 9 (clCASP9) (B, D) in the developing mouse frontal limb at prenatal day E15. Both proteins were detected in consecutive sections. Positive signal in green (arrows), nuclei counterstained with DAPI (blue); total magnification ×20 (A, B) or ×40 (C, D). hc, hypertrophic cartilage.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Immunofluorescent staining of ABHD2 (A, C) and cleaved caspase 9 (clCASP9) (B, D) in the developing mouse frontal limb at prenatal day E15. Both proteins were detected in consecutive sections. Positive signal in green (arrows), nuclei counterstained with DAPI (blue); total magnification ×20 (A, B) or ×40 (C, D). hc, hypertrophic cartilage.

    Article Snippet: Consecutive sections were pretreated in citrate buffer (10 min/98 °C) for antigen retrieval and then incubated with ABHD2 antibody (14039-1-AP, Proteintech, Germany) or antibody specific to cleaved CASP-9 (9509, Cell Signaling Technology, USA) overnight.

    Techniques: Staining

    Proteins Acting as Negative Regulators of Cell Migration Upregulated after Casp9 KO in Both Clones Compared to Mock and wt Cells <xref ref-type= a " width="100%" height="100%">

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Proteins Acting as Negative Regulators of Cell Migration Upregulated after Casp9 KO in Both Clones Compared to Mock and wt Cells a

    Article Snippet: Consecutive sections were pretreated in citrate buffer (10 min/98 °C) for antigen retrieval and then incubated with ABHD2 antibody (14039-1-AP, Proteintech, Germany) or antibody specific to cleaved CASP-9 (9509, Cell Signaling Technology, USA) overnight.

    Techniques: Migration, Clone Assay

    (A) Protein and (B) mRNA levels of ABHD2 and ADAM15 in parental, mock, and Casp9 KO MC3T3-E1 cells; α-tubulin was used as a loading control for immunoblotting. Data represents means ± SD from at least three independent experiments.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: (A) Protein and (B) mRNA levels of ABHD2 and ADAM15 in parental, mock, and Casp9 KO MC3T3-E1 cells; α-tubulin was used as a loading control for immunoblotting. Data represents means ± SD from at least three independent experiments.

    Article Snippet: Blots were probed with CASP-9 (#9508, Cell Signaling Technology, USA), cleaved CASP-3 (#9661, Cell Signaling Technology, USA), ABHD2 (14039-1-AP, Proteintech, Germany), ADAM15 (GTX101599, GeneTex, USA), BST-2 (sc-390719, Santa Cruz Biotechnology, USA; 13560-1-AP, Proteintech, Germany; #60066S, Cell Signaling Technology, USA), or α-tubulin (ab7291, Abcam, UK) specific antibodies and horseradish peroxidase-conjugated mouse or rabbit secondary antibodies (Sigma-Aldrich, USA).

    Techniques: Control, Western Blot

    Protein levels of ABHD2 and ADAM15 in MC3T3-E1 cells with inhibited CASP-9 or CASP-3/-7; α-tubulin was used as a loading control.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Protein levels of ABHD2 and ADAM15 in MC3T3-E1 cells with inhibited CASP-9 or CASP-3/-7; α-tubulin was used as a loading control.

    Article Snippet: Blots were probed with CASP-9 (#9508, Cell Signaling Technology, USA), cleaved CASP-3 (#9661, Cell Signaling Technology, USA), ABHD2 (14039-1-AP, Proteintech, Germany), ADAM15 (GTX101599, GeneTex, USA), BST-2 (sc-390719, Santa Cruz Biotechnology, USA; 13560-1-AP, Proteintech, Germany; #60066S, Cell Signaling Technology, USA), or α-tubulin (ab7291, Abcam, UK) specific antibodies and horseradish peroxidase-conjugated mouse or rabbit secondary antibodies (Sigma-Aldrich, USA).

    Techniques: Control

    Immunofluorescent staining of ABHD2 (A, C) and cleaved caspase 9 (clCASP9) (B, D) in the developing mouse frontal limb at prenatal day E15. Both proteins were detected in consecutive sections. Positive signal in green (arrows), nuclei counterstained with DAPI (blue); total magnification ×20 (A, B) or ×40 (C, D). hc, hypertrophic cartilage.

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Immunofluorescent staining of ABHD2 (A, C) and cleaved caspase 9 (clCASP9) (B, D) in the developing mouse frontal limb at prenatal day E15. Both proteins were detected in consecutive sections. Positive signal in green (arrows), nuclei counterstained with DAPI (blue); total magnification ×20 (A, B) or ×40 (C, D). hc, hypertrophic cartilage.

    Article Snippet: Blots were probed with CASP-9 (#9508, Cell Signaling Technology, USA), cleaved CASP-3 (#9661, Cell Signaling Technology, USA), ABHD2 (14039-1-AP, Proteintech, Germany), ADAM15 (GTX101599, GeneTex, USA), BST-2 (sc-390719, Santa Cruz Biotechnology, USA; 13560-1-AP, Proteintech, Germany; #60066S, Cell Signaling Technology, USA), or α-tubulin (ab7291, Abcam, UK) specific antibodies and horseradish peroxidase-conjugated mouse or rabbit secondary antibodies (Sigma-Aldrich, USA).

    Techniques: Staining

    Proteins Acting as Negative Regulators of Cell Migration Upregulated after Casp9 KO in Both Clones Compared to Mock and wt Cells <xref ref-type= a " width="100%" height="100%">

    Journal: Journal of Proteome Research

    Article Title: Caspase-9 Is a Positive Regulator of Osteoblastic Cell Migration Identified by diaPASEF Proteomics

    doi: 10.1021/acs.jproteome.3c00641

    Figure Lengend Snippet: Proteins Acting as Negative Regulators of Cell Migration Upregulated after Casp9 KO in Both Clones Compared to Mock and wt Cells a

    Article Snippet: Blots were probed with CASP-9 (#9508, Cell Signaling Technology, USA), cleaved CASP-3 (#9661, Cell Signaling Technology, USA), ABHD2 (14039-1-AP, Proteintech, Germany), ADAM15 (GTX101599, GeneTex, USA), BST-2 (sc-390719, Santa Cruz Biotechnology, USA; 13560-1-AP, Proteintech, Germany; #60066S, Cell Signaling Technology, USA), or α-tubulin (ab7291, Abcam, UK) specific antibodies and horseradish peroxidase-conjugated mouse or rabbit secondary antibodies (Sigma-Aldrich, USA).

    Techniques: Migration, Clone Assay